identification of highly specific scfvs against total adiponectin for diagnostic purposes

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ID: 169117
2017
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Abstract
Adiponectin is one of the most abundant adipokines secreted from adipose tissue. It acts as an endogenous insulin sensitizer and plasma concentrations are inversely correlated with obesity and metabolic syndrome. A decrease in plasma adiponectin levels normally indicates increased hormonal activity of the visceral lipid tissue, which is associated with decreased insulin sensitivity. It may therefore be considered a valuable biomarker for elucidating the underlying deteriorations resulting in type 2 diabetes and macrovascular disease. Here we present the use of phage display technology to identify highly specific antibody fragments (scFvs) against adiponectin. The selected scFvs showed highly specific binding to globular and native adiponectin in ELISA tests. By using our phage display technology, we were able to obtain monoclonal antibodies with specific high affinity binding to the target protein in an effective and easy to upscale manner. The selected scFvs against adiponectin can be used for developing immunoassays suitable for use in metabolic syndrome diagnosis and monitoring.
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wilton2017biologyidentification Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Peter Wilton;Michael Steidel;Gabriele Krczal;Iris Hermanns;Andreas Pfützner;Alisa Konnerth;Kajohn Boonrod
Journal Journal of microencapsulation
Year 2017
DOI
10.3390/biology6020026
URL
http://www.mdpi.com/2079-7737/6/2/26
Keywords
adiponectin phage display scfv

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