electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic sod, catalase in an in vitro assay system by pectic polysaccharides of momordica charantia in combination with curcumin
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2017
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Abstract
Momordica charantia (MC) fruit known as bitter gourd, is of potential nutritional and medicinal value. The objectives of the present in vitro study were to evaluate the efficacy of bioactive pectic polysaccharides (CCPS) of MC along with another well-known bioactive compound curcumin in the abrogation of hepatocellular oxidative stress persuaded by sodium arsenite. Electrozymographic method was developed for the assessment of superoxide dismutase (SOD) and catalase activities of liver tissues maintained under an in vitro system. A significant association of CCPS of MC in combination with curcumin was found in the alleviation of oxidative stress induced by sodium arsenite in liver slice. Generated data pointed out that CCPS of MC and curcumin separately or in combination can offer significant protection against alterations in malondialdehyde (MDA), conjugated diene (CD) and antioxidative defense (SOD, CAT) markers. Furthermore, results of hepatic cell DNA degradation strongly supported that both these co-administrations have efficacy in preventing cellular damage. This is the first information of extracted polysaccharides from MC preventing arsenic induced damage in a liver slice of rat.
| Reference Key |
perveen2017biochemistryelectrozymographic
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| Authors | ;Hasina Perveen;Moumita Dash;Shamima Khatun;Moulima Maity;Syed Sirajul Islam;Sandip Chattopadhyay |
| Journal | addictive behaviors reports |
| Year | 2017 |
| DOI |
10.1016/j.bbrep.2017.06.002
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