antibiofilm effect of dnase against single and mixed species biofilm

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ID: 166840
2018
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Abstract
Biofilms are aggregates of microorganisms that coexist in socially coordinated micro-niche in a self-produced polymeric matrix on pre-conditioned surfaces. The biofilm matrix reduces the efficacy of antibiofilm strategies. DNase degrades the extracellular DNA (e-DNA) present in the matrix, rendering the matrix weak and susceptible to antimicrobials. In the current study, the effect of DNase I was evaluated during biofilm formation (pre-treatment), on preformed biofilms (post-treatment) and both (dual treatment). The DNase I pre-treatment was optimized for P. aeruginosa PAO1 (model biofilm organism) at 10 µg/mL and post-treatment at 10 µg/mL with 15 min of contact duration. Inclusion of Mg2+ alongside DNase I post-treatment resulted in 90% reduction in biofilm within only 5 min of contact time (irrespective of age of biofilm). On extension of these findings, DNase I was found to be less effective against mixed species biofilm than individual biofilms. DNase I can be used as potent antibiofilm agent and with further optimization can be effectively used for biofilm prevention and reduction in situ.
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sharma2018foodsantibiofilm Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Komal Sharma;Ankita Pagedar Singh
Journal journal of biological chemistry
Year 2018
DOI
10.3390/foods7030042
URL
http://www.mdpi.com/2304-8158/7/3/42
Keywords
biofilms post-treatment pre-treatment dnase i antibiofilm methodschemical technology

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