generation of a clonal induced pluripotent stem cell (ipsc) line expressing the mutant mecp2 allele from a rett syndrome patient fibroblast line
Clicks: 182
ID: 166435
2017
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
182 views
14 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #74 of 117 articles by views in journal of energy chemistry
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Human fibroblast cells collected from a 3-year old, female Rett Syndrome patient with a 32 bp deletion in the X-linked MECP2 gene were obtained from the Coriell Institute. Fibroblasts were reprogrammed to iPSC cells using a Sendai-virus delivery system expressing human KOSM transcription factors. Cell-line pluripotency was demonstrated by gene expression, immunocytochemistry, in-vitro differentiation trilineage capacity and was of normal karyotype. Interestingly, subsequent clones retained the epigenetic memory of the parent fibroblasts allowing for the segregation of wild-type and mutant expressing clones. This MECP2 mutant expressing clone may serve as a model for investigating MECP2 reactivation in Rett's Syndrome.
| Reference Key |
hunihan2017stemgeneration
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Lisa Hunihan;Jeffrey Brown;Angela Cacace;Alda Fernandes;Andrea Weston |
| Journal | journal of energy chemistry |
| Year | 2017 |
| DOI |
10.1016/j.scr.2017.02.017
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.