influência da qualidade de luz e silício no crescimento in vitro de orquídeas nativas e híbridas light quality and silicon on growth in vitro of native and hybrid orchid species

Clicks: 278
ID: 166415
2011
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Abstract
Objetivou-se avaliar a influência do ambiente de cultivo e de concentrações de silicato de cálcio no crescimento in vitro de uma espécie nativa de orquídea (Brassavola perrine) e de um híbrido [(Laelia cattleya Culminant "Tuilerie" x Laelia cattleya Sons Atout Rotunda) x Brassolaelia cattleya Startifire Moon Beach]. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 0,5 cm de comprimento foram inoculadas em frascos com capacidade para 250 mL contendo 60 mL de meio de cultura MS, acrescido de silicato de cálcio (0; 0,5; 1,0 e 2,0 mg L-1) em ambientes de cultivo [natural (casa de vegetação) e artificial (em sala de crescimento)], em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8±0,1 e geleificado com 5,5 g L-1 de ágar antes do processo de autoclavagem a 121ºC e 1 atm por 20 minutos. Ao final de 150 dias foram realizadas avaliações biométricas e análises micromorfológicas (miscroscopia de varredura) nas plântulas. Maior crescimento foi obtido com a utilização de 2,0 mg L-1 e 1,0 mg L-1 de silicato de cálcio para o híbrido e espécie nativa, respectivamente, em sala de crescimento. As orquídeas em estudo são espécies anfiestomáticas, com estômatos do tipo tetracítico (híbrida) e anomocítico (nativa). A presença do silício na estrutura foliar proporcionou o correto desenvolvimento (ausência de deformações estruturais) de plântulas de orquídeas.
The vegetative propagation techniques using tissue culture can be a valuable tool in the production of orchid seedlings. The objective of this study was to determine the concentration of calcium silicate and light quality that provides better growth in seedlings of native orchid (Brassavola perrine) and hybrid [(Laelia cattleya Climax "Tuilerie" x Laelia cattleya Sons Atout Rotunda) x Brassolaelia cattleya Startifire Moon Beach]. Seedlings from seeds germinated in vitro with approximately 0.5 cm long were inoculated in 250 mL flasks containing 60 mL of culture medium MS, plus calcium silicate (0, 0.5, 1.0 and 2.0 mg L-1) in environments of culture [natural (a greenhouse) and artificial (in a growth room)] in all possible combinations. The culture medium had its pH adjusted to 5.8 ± 0.1 and solidified with 5.5 g L-1 of agar before autoclaving at 121º C and 1 atm for 20 minutes. After 150 days biometrics and ultrastructural analysis (scanning microscopy) were carried out. Greater growth of seedlings was obtained with the use of 2.0 mg L-1 and 1.0 mg L-1 of calcium silicate for hybrid and native species, respectively. The orchid species in study are anfistomatic with the type tetracytic (hybrid) and anomocytic (native) stomata. The presence of silicon in the leaf structure provided correct development (absence of structural deformations) of seedlings of orchids.
Reference Key
pasqual2011horticulturainfluncia Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Moacir Pasqual;Joyce DR Soares;Filipe A Rodrigues;Aparecida G de Araujo;Roseneide R dos Santos
Journal research on biomedical engineering
Year 2011
DOI
10.1590/S0102-05362011000300011
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000300011
Keywords
microscopy in vitro culture biometricsplant culture

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