dna- binding, biological activities and chemical composition of wild growing epilobium angustifolium l. extracts from canakkale, turkey

The usage of Epilobium angustifolium L. in folk medicine against prostate and skin diseases encouraged us to investigate pBR322 DNA-binding ability, biological activities and chemical composition of wild growing Epilobium angustifolium L.’s methanolic, ethanolic and aqueous extracts from Canakkale, Turkey. DNA-binding activity was studied by agarose gel electrophoresis and chemical composition via GC, GC-MS, HPLC, LC-MS, ICP-MS and AAS. Literature survey indicates no data on pBR322 plasmid DNA interaction of Epilobium species which is an important criteria for being a potential anticancer agent. Antibacterial and antifungal effects were tested against 11 bacterias and 3 fungis and compared with ampicillin, chloramphenicol and ketokanozol by agar well diffusion techniques. Total phenolic content was found by Folin-Ciocalteu method and antioxidant activity was obtained by DPPH and Fe (II)-FZ (ferric-ferrozine) methods. This plant is rich of flavonoids, hormones, fatty and phenolic acids. Si, K, Mg, P and Mn were the main inorganic elements. Epilobium angustifolium L. with its high DNA-binding activity and rich chemical composition can be a good potential anticancer, antimicrobial and antioxidant prodrug.