modification of cɛ mrna expression by ebv-encoded latent membrane protein 1
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ID: 164635
2006
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Abstract
To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by
Epstein-Barr virus (EBV) on Cɛ mRNA expression,
mRNA levels were examined by RT-PCR
or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from
Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris
suum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1,
using lipofection method. Cɛ mRNA levels were
considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from
the nonimmunized rats; however, Cɛ mRNA levels were
decreased in the splenocytes derived from the immunized rats. Cɛ
mRNA expression in IgE-producing cells are modulated by LMP1, which might depend on
the differentiation status of B cells upon exposure to allergen.
| Reference Key |
hanashiro2006mediatorsmodification
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|---|---|
| Authors | ;Kazuhiko Hanashiro;Shigeto Ohta;Masanori Sunagawa;Mariko Nakamura;Mikio Suzuki;Tadayoshi Kosugi |
| Journal | polyhedron |
| Year | 2006 |
| DOI |
10.1155/MI/2006/68069
|
| URL | |
| Keywords |
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