novel strategies for expansion of tooth epithelial stem cells and ameloblast generation

Clicks: 110
ID: 163090
2020
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Abstract
Abstract Enamel is secreted by ameloblasts derived from tooth epithelial stem cells (SCs). Humans cannot repair or regenerate enamel, due to early loss of tooth epithelial SCs. Contrarily in the mouse incisors, epithelial SCs are maintained throughout life and endlessly generate ameloblasts, and thus enamel. Here we isolated Sox2-GFP+ tooth epithelial SCs which generated highly cellular spheres following a novel in vitro strategy. This system enabled analysis of SC regulation by various signaling molecules, and supported the stimulatory and inhibitory roles of Shh and Bmp, respectively; providing better insight into the heterogeneity of the SCs. Further, we generated a novel mouse reporter, Enamelin-tdTomato for identification of ameloblasts in live tissues and cells, and used it to demonstrate presence of ameloblasts in the new 3D co-culture system of dental SCs. Collectively, our results provide means of generating 3D tooth epithelium from adult SCs which can be utilized toward future generation of enamel.
Reference Key
binder2020scientificnovel Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Martin Binder;Leah C. Biggs;Mark S. Kronenberg;Pascal Schneider;Irma Thesleff;Anamaria Balic
Journal historia de la educación
Year 2020
DOI
10.1038/s41598-020-60708-w
URL
http://link.springer.com/article/10.1038/s41598-020-60708-w
Keywords
medicinescience

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