effects of culture substrate made of poly(n-isopropylacrylamide-co-acrylic acid) microgels on osteogenic differentiation of mesenchymal stem cells

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ID: 162870
2016
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Abstract
Poly(N-isopropylacrylamide) (PNIPAM)-based polymers and gels are widely known and studied for their thermoresponsive property. In the biomaterials category, they are regarded as a potential cell culture substrate, not only because of their biocompatibility, but also their special character of allowing controlled detachment of cells via temperature stimulus. Previous research about PNIPAM-based substrates mostly concentrated on their effects in cell adhesion and proliferation. In this study, however, we investigate the influence of the PNIPAM-based substrate on the differentiation capacity of stem cells. Especially, we choose P(NIPAM-AA) microgels as a culture dish coating and mesenchymal stem cells (MSCs) are cultured on top of the microgels. Interestingly, we find that the morphology of MSCs changes remarkably on a microgel-coated surface, from the original spindle form to a more stretched and elongated cell shape. Accompanied by the alternation in morphology, the expression of several osteogenesis-related genes is elevated even without inducing factors. In the presence of full osteogenic medium, MSCs on a microgel substrate show an enhancement in the expression level of osteopontin and alizarin red staining signals, indicating the physical property of substrate has a direct effect on MSCs differentiation.
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dai2016moleculeseffects Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhuojun Dai, Yinglan Shu, Chao Wan, Chi Wu
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Journal Journal of ethnopharmacology
Year 2016
DOI
10.3390/molecules21091192
URL
http://www.mdpi.com/1420-3049/21/9/1192
Keywords
mesenchymal stem cells cell morphology extracellular matrix microgels

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