a corrosive oesophageal burn model in rats: double-lumen central venous catheter usage
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ID: 162796
2015
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Abstract
Background: We aimed to create a new and less invasive experimental corrosive oesophageal burn model using a catheter without a gastric puncture (gastrotomy). Materials and Methods: We conducted the study with two groups composed of 8 male rats. The experimental oesophageal burn was established by the application of 10% sodium hydroxide to the distal oesophagus under a pressure of 20 cmH 2 O, via 5-F double-lumen central venous catheter without a gastrotomy. The control group was given 0.9% sodium chloride. All rats were killed 24 h after administration of NaOH or 0.9% NaCl. Histologic damage to oesophageal tissue was scored by a single pathologist blind to groups. Results: The rats in the control group were observed to have no pathological changes. Corrosive oesophagitis (tissue congestion, oedema, inflammation, ulcer and necrosis) was observed in rats exposed to NaOH. Conclusion: We believe that an experimental corrosive oesophageal burn can safely be created under same hydrostatic pressure without a gastric puncture using this model.
| Reference Key |
bakan2015africana
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|---|---|
| Authors | ;Vedat Bakan;Harun Çiralik;Seyfi Kartal |
| Journal | intelligent systems reference library |
| Year | 2015 |
| DOI |
10.4103/0189-6725.172560
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