construction and characterization of a glycoprotein e deletion mutant of bovine herpesvirus type 1.2 strain isolated in brazil construção e caracterização de uma amostra de bohv-1.2 isolada no brasil com uma deleção no gene da glicoproteína e

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ID: 162032
2002
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Abstract
This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co-transfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine.
Este artigo descreve a construção e caracterização de uma amostra de um herpesvírus bovino tipo 1.2a (BoHV-1.2a) que apresenta uma deleção na região genômica que codifica a glicoproteína E (gE). A deleção gênica foi induzida através da co-transfecção de um fragmento de deleção, contendo as regiões 5´e 3´flanqueadoras da gE, com o DNA viral intacto de uma amostra viral isolada de um animal que apresentava doença respiratória. O isolamento do vírus gE negativo (gE-) foi realizado com auxílio da técnica de imunoperoxidase em que foi utilizado como anticorpo primário um anticorpo monoclonal anti-gE. O vírus gE- foi purificado e o DNA isolado desta amostra foi examinado através das técnicas de análise por enzimas de restrição e "Southern blot". Esta amostra gE- será avaliada como candidata para compor uma vacina diferencial contra a rinotraqueíte infecciosa dos bovinos.
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franco2002brazilianconstruction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ana C. Franco;Frans A.M. Rijsewijk;Eduardo F. Flores;Rudi Weiblen;Paulo M. Roehe
Journal ieee software
Year 2002
DOI
10.1590/S1517-83822002000300018
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300018
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