delivery of human ev71 receptors by adeno-associated virus increases ev71 infection-induced local inflammation in adult mice

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2014
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Abstract
Enterovirus71 (EV71) is now recognized as an emerging neurotropic virus in Asia and one major causative agent of hand-foot-mouth diseases (HFMD). However potential animal models for vaccine development are limited to young mice. In this study, we used an adeno-associated virus (AAV) vector to introduce the human EV71 receptors P-selectin glycoprotein ligand-1 (hPSGL1) or a scavenger receptor class-B member-2 (hSCARB2) into adult ICR mice to change their susceptibility to EV71 infection. Mice were administered AAV-hSCARB2 or AAV-hPSGL1 through intravenous and oral routes. After three weeks, expression of human SCARB2 and PSGL1 was detected in various organs. After infection with EV71, we found that the EV71 viral load in AAV-hSCARB2- or AAV-hPSGL1-transduced mice was higher than that of the control mice in both the brain and intestines. The presence of EV71 viral particles in tissues was confirmed using immunohistochemistry analysis. Moreover, inflammatory cytokines were induced in the brain and intestines of AAV-hSCARB2- or AAV-hPSGL1-transduced mice after EV71 infection but not in wild-type mice. However, neurological disease was not observed in these animals. Taken together, we successfully infected adult mice with live EV71 and induced local inflammation using an AAV delivery system.
Reference Key
hsiao2014biomeddelivery Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hung-Bo Hsiao, Ai-Hsiang Chou, Su-I Lin, Shu-Pei Lien, Chia-Chyi Liu, Pele Chong, Chih-Yeh Chen, Mi-Hua Tao, Shih-Jen Liu
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Journal spectrochimica acta - part a: molecular and biomolecular spectroscopy
Year 2014
DOI
10.1155/2014/878139
URL
http://dx.doi.org/10.1155/2014/878139
Keywords
Medicine

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