efeito da uréia no alongamento e enraizamento de microplantas de bananeira in vitro effect of urea on in vitro growth and rooting of banana microplants

Clicks: 272
ID: 161215
2009
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Abstract
A micropropagação de bananeira (Musa spp.) via ápices caulinares já está bem estabelecida para diversas cultivares. Entretanto, podem ser feitos aperfeiçoamentos nos protocolos visando a obtenção de mudas mais vigorosas. Objetivou-se, neste trabalho, verificar a adição de nitrogênio na forma de uréia em diferentes concentrações (0, 100, 200, 400 e 800 mg L-1), no alongamento e enraizamento de brotos das cultivares Grande Naine, Prata Anã, Maçã e Nanicão. Houve efeito positivo da uréia no crescimento dos explantes, na produção de raízes e de massa seca para a cultivar Maçã, até 200 mg L-1 de uréia. As cultivares Prata Anã e Grande Naine não foram beneficiadas com a adição de uréia, e a concentração de 800 mg L-1 gerou morte das microplantas dessas cultivares e da cultivar Maçã. 'Nanicão' foi mais tolerante às altas concentrações de uréia, e a concentração de 800 mg L-1 foi ótima para seu desenvolvimento. Além disso, a uréia induziu brotações em 'Nanicão' nas concentrações mais baixas.
Banana (Musa spp.) micropropagation via shoot tips has already been established for many cultivars. However, protocol adjustments aiming to obtain more vigorous plants can be made. The aim of this work was to verify the nitrogen addition in the form of urea on different concentrations (0, 100, 200, 400 and 800 mg L-1) on shoot growth and rooting of Grande Naine, Prata Anã, Maçã and Nanicão cultivars. There was a positive urea effect on Maçã shoot growth, dry matter production and rooting up to 200 mg L-1 concentrations. Prata Anã and Grande Naine cultivars were not benefited by urea addition, while 800 mg L-1 concentration lead to death of microplants. However, Nanicão cultivar was more tolerant to higher urea concentrations, and 800 mg L-1 was optimal for its development. Moreover, urea induced shoot growth on 'Nanicão' at lower concentrations.
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Authors ;Rachel Soares Ramos;Sérgio Yoshimitsu Motoike;Elisa Ferreira Moura;Simone Bhering de Souza Gomes;Vânia de Fátima Rodrigues;Márcio Antonio Rocha de Oliveira
Journal information and software technology
Year 2009
DOI
10.1590/S1413-70542009000700024
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700024
Keywords
cell culture nitrogen sourcesagriculture (general)

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