continuous recirculation of microdroplets in a closed loop tailored for screening of bacteria cultures

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ID: 158597
2018
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Abstract
Emerging microfluidic technology has introduced new precision controls over reaction conditions. Owing to the small amount of reagents, microfluidics significantly lowers the cost of carrying a single reaction. Moreover, in two-phase systems, each part of a dispersed fluid can be treated as an independent chemical reactor with a volume from femtoliters to microliters, increasing the throughput. In this work, we propose a microfluidic device that provides continuous recirculation of droplets in a closed loop, maintaining low consumption of oil phase, no cross-contamination, stabilized temperature, a constant condition of gas exchange, dynamic feedback control on droplet volume, and a real-time optical characterization of bacterial growth in a droplet. The channels (tubing) and junction cubes are made of Teflon fluorinated ethylene propylene (FEP) to ensure non-wetting conditions and to prevent the formation of biofilm, which is particularly crucial for biological experiments. We show the design and operation of a novel microfluidic loop with the circular motion of microdroplet reactors monitored with optical sensors and precision temperature controls. We have employed the proposed system for long term monitoring of bacterial growth during the antibiotic chloramphenicol treatment. The proposed system can find applications in a broad field of biomedical diagnostics and therapy.
Reference Key
debski2018micromachinescontinuous Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Pawel R. Debski;Karolina Sklodowska;Jacek A. Michalski;Piotr M. Korczyk;Miroslaw Dolata;Slawomir Jakiela
Journal chemistryopen
Year 2018
DOI
10.3390/mi9090469
URL
http://www.mdpi.com/2072-666X/9/9/469
Keywords
Screening antibiotic treatment bacteria cultures microfluidic loop

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