fetuin: a tool to study the block to polyspermy fetuina: um instrumento para estudar o bloqueio da polispermia

Clicks: 183
ID: 156435
1997
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Abstract
The effect of fetuin, an a1-glycoprotein that has protease inhibitor activity, on the biock to potyspermy was determined. Cumulus-oocyte complexes from eCG primed mice were matured in vitro in the presence of 0, 0.01, 0.1, l and 10mg/ml of fetuin in modifica TCM 199. Both in vivo and in vitro matured oocytes were fertilized in the presence of fetuin and incubated for 6 and 24h. Fetuin present in a concentration of 1 mg/ml in the ferlilization but not in the maturation medium was able to induce polyspermy in 52.4% of the eggs. There was a positive relationship between concentration of fetuin in the fertilization medium and the proportion of polyspermic eggs (p<0.05). A significant interaclion between 0.1 and l mg/ml of fetuin during maturation and fertilization was observed (p<0.05). The results of these experiments demonstrate the inhibition of the block to polyspermy using a protease inhibitor during the fertilization.
No presente experimento, foi determinado o efeito da fetuina, uma a1-glicoproteina com capacidade de inibir protease, no bloqueio da polispermia. Complexos cumulus-oócitos de murideos superovulados com eCG foram maturados in vitro na presença de 0, 0,01. 0,1, lê Idmg/ml de fetuina em TCM 199 modificado. Oócitos maturados in vivo e in vitro foram fecundados na presença de fetuina e incubados por 6 e 24 horas. Fetuina presente em uma concentração de 1 mg/ml durante a fecundação. mas não no meio de maturação, foi capaz de induzir polispermia em 52,5% dos óvulos. Houve uma relação positiva entre u concentração de fetuina no meio de fecundação e a percentagem de óvulos polispérmicos (p<0.05). Uma interação significativa entre 0,1 e l,0mg/ml de fetuina durante a maturação e fecundação foi observada (p<0.05). Os resultados destes experimentos demonstram a inibição do bloqueio da polispermia utilizando um inibidor de protease durante a fecundação.
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gonalves1997cinciafetuin: Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Paulo Bayard Gonçalves;Charles Graves
Journal Trials
Year 1997
DOI
10.1590/S0103-84781997000100022
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000100022
Keywords
fetuin

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