aminoglycoside resistance profile and structural architecture of the aminoglycoside acetyltransferase aac(6’)-im

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ID: 155713
2017
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Abstract
Aminoglycoside 6’-acetyltransferase-Im (AAC(6’)-Im) is the closest monofunctional homolog of the AAC(6’)-Ie acetyltransferase of the bifunctional enzyme AAC(6’)-Ie/APH(2”)-Ia. The AAC(6’)-Im acetyltransferase confers 4- to 64-fold higher MICs to 4,6-disubstituted aminoglycosides and the 4,5-disubstituted aminoglycoside neomycin than AAC(6’)-Ie, yet unlike AAC(6’)-Ie, the AAC(6’)-Im enzyme does not confer resistance to the atypical aminoglycoside fortimicin. The structure of the kanamycin A complex of AAC(6’)-Im shows that the substrate binds in a shallow positively-charged pocket, with the N6’ amino group positioned appropriately for an efficient nucleophilic attack on an acetyl-CoA cofactor. The AAC(6’)-Ie enzyme binds kanamycin A in a sufficiently different manner to position the N6’ group less efficiently, thereby reducing the activity of this enzyme towards the 4,6-disubstituted aminoglycosides. Conversely, docking studies with fortimicin in both acetyltransferases suggest that the atypical aminoglycoside might bind less productively in AAC(6’)-Im, thus explaining the lack of resistance to this molecule.
Reference Key
smith2017microbialaminoglycoside Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Clyde A. Smith;Monolekha Bhattacharya;Marta Toth;Nichole K. Stewart;Sergei B. Vakulenko
Journal povolžskaâ arheologiâ
Year 2017
DOI
10.15698/mic2017.12.602
URL
http://microbialcell.com/researcharticles/aminoglycoside-resistance-profile-and-structural-architecture-of-the-aminoglycoside-acetyltransferase-aac6-im/
Keywords
antibiotic resistance aminoglycosides acetyltransferase crystal structurebiology (general)

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