efeito da natação associado a diferentes dietas sobre o músculo tibial anterior do rato: estudo morfológico e histoquímico efecto de la natación asociado a diferentes dietas sobre el músculo tibial anterior del ratón: estudio morfológico e histo-químico effect of swimming associated with diet on the anterior tibial muscle of rats: morphological and hystochemical study
تأثير السباحة المقترنة بحميات غذائية مختلفة على العضلة الظنبوبية الأمامية لدى الجرذ: دراسة مورفولوجية وكيميائية نسيجية؛ تأثير السباحة المقترن بحميات غذائية مختلفة على العضلة الظنبوبية الأمامية لدى الفأر: دراسة مورفولوجية وكيميائية نسيجية؛ تأثير السباحة المقترنة بالنظام الغذائي على العضلة الظنبوبية الأمامية لدى الجرذان: دراسة مورفولوجية وكيميائية نسيجية.
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Abstract
El objetivo de ese estudio fue investigar el efecto de la asociación de frecuencias diferentes de programas de natación y dietas en las características del músculo tibial anterior. Se usaron 24 ratones masculinos Wistar. Éstos fueron aleatoriamente divididos en 3 grupos: no especializado (control), el grupo especializado (2 x/sem) y el grupo 2 especializado (5 x/sem). Cada grupo era dividido en 2 grupos que recibieron uno de las dietas: normal o hipercalórica. Después del periodo de entrenamiento las muestras del músculo fueron recogidas y reunido congeladas a -70ºC. Los cortes histológicos (8µm) obtenidos con un micrótomo de criostato (-20º) los mismos fueron sometidos a los métodos HE y NADH-TR, m-ATPase (pH 4,4) y Negro de Sudán. Se estimaron la morfología y el grado de la hipertrofia usando el método del diámetro más pequeño. Los datos se sometieron a prueba variable (ANOVA-one way). Las fibras musculares fueron clasificadas como SÓ, FOG y FG, presentando una distribución "de modelo en mosaico" en todos los grupos. Las fibras musculares revelaron una hipertrofia ligera en todos los grupos. Los pesos inicial y final fueron significativamente diferentes en los grupos especializados. Sobre todo en el grupo de 5 x/sem, de los grupos especializados, las fibras musculares revelaron hipertrofia, splitting, e con algunos mionúcleos internos. Algunas fibras se revelaron atróficas, y esta observación hizo pensar en una denervación. La oxidación del metabolismo SÓ era más intensa en las fibras y en el FOG; no había alteraciones en la habilidad contráctil del músculo y el tenor de lípidos en el grupo SÓ era intenso en las fibras, moderado en el FOG y débil en FG. El estudio presente, con este protocolo, en los grupos entrenados de 2 x/sem y 5 x/sem, causó tipos diferentes de lesiones morfológicas en las fibras. Los de dieta hipercalórica no provocaron resultados estadísticamente significativos comparados con los de la dieta normal.
The objective of this study was to investigate the effect of the association of different swimming program frequencies and diets on the characteristics of the anterior tibial muscle of 24 Wistar male rats. These rats were randomly assigned into three groups: untrained (control), trained group (two days/week) and trained group (five days/week). Each group was divided into two groups, which received one of the two normal or high calorie diets. After the training period, muscle samples were collected and frozen at -70ºC. Serial cryostat sections (8 mum) were sectioned and submitted to HE stain and to NADH-TR, m-ATPase (pH 4.4) and Sudan Black histochemical methods. The morphology was analyzed and the degree of fiber enlargement (on hypertrophy) evaluated using the lesser fiber diameter method. The data were submitted to analysis of variance (one way ANOVA). Muscle fibers were classified as SO, FOG and FG, presenting a mosaic distribution pattern, which were unchanged in all groups. Muscle fibers revealed a very low hypertrophy in all groups. Initial and final body weight were significantly different in trained groups. In the trained groups, especially in five days/week, muscle fibers revealed higher diameter, splitting and some internal myonucleus. Some atrophic fibers were observed and this observation was suggestive of denervation. The oxidative metabolism was higher in SO and FOG fibers. No significant alterations were observed in muscle contraction ability and the lipids content was intense in SO fibers, moderate in FOG fibers and low in FG fibers. The present study, with this protocol of two days/week and five days/week training caused different types of morphological lesions in fibers. The high calorie diet did not cause statistically significant results in comparison with the normal diet.
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ciabattari2005revistaefeito
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| Authors | ;Olimpia Ciabattari;Alexandre Dal Pai;Vitalino Dal Pai |
| Journal | research disclosure |
| Year | 2005 |
| DOI |
10.1590/S1517-86922005000200005
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