pelacakan gen env-tm virus penyakit jembrana galur tabanan 1995 dengan metode nucleic acid sequence based amplificaton

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ID: 154475
2016
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Abstract
Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.
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kusumawati2016jurnalpelacakan Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Asmarani Kusumawati;Atik Ratnawati;Ida Arlita Wulandari;Sri Hartati;Tri Untari
Journal journal of advanced research
Year 2016
DOI
DOI not found
URL
https://ojs.unud.ac.id/index.php/jvet/article/view/17340
Keywords
tabanan 1995 strainveterinary medicine

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