cell death differentiation in black headed rams spermatozoa, using fluorescent labeled annexin v

Clicks: 320
ID: 153686
2008
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Abstract
Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered translocation of PS in treated with dexamethazone groups at the 10-th min and in control no treated varied from 2.74%±0.65 to 2.30%±0.89. After the 5 hour of incubation these % increased to 39.83±3.33 for the treated group and 23.44±1.12 for the control. It was concluded that Annexin V binding assay is more sensitive in the detection of deterioration in membrane function than other conventional methods such as motility analysis and supravital techniques.
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ivanova-kicheva2008journalcell Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors M Ivanova-Kicheva, A Kukov, D Daskalova, E Solenkova, I Nikolov, L Todorov
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Journal balkan medical journal
Year 2008
DOI
DOI not found
URL
http://jcea.agr.hr/articles/517_CELL_DEATH_DIFFERENTIATION_IN_BLACK_HEADED_RAMS_SPERMATOZOA_USING_FLUORESCENT_LABELED_ANNEXIN_V_en.pdf
Keywords
spermatozoa plasma membrane phosphatidylserineagriculture

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