cellular hnrnp a1 interacts with nucleocapsid protein of porcine epidemic diarrhea virus and impairs viral replication

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ID: 153106
2018
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Abstract
The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells. Co-immunoprecipitation (CO-IP) results clearly demonstrated that PEDV N protein could bind to human hnRNP A1. Replication of PEDV was inhibited by silencing the expression of hnRNP A1 in CCL-81 cells, suggesting the positive effect of hnRNP A1 on PEDV infection.
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Authors ;Zhonghua Li;Wei Zeng;Shiyi Ye;Jian Lv;Axiu Nie;Bingzhou Zhang;Yumei Sun;Heyou Han;Qigai He
Journal International journal of pharmaceutics
Year 2018
DOI
10.3390/v10030127
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