optimization of extraction parameters for recovery of a-amylase from the fermented bran of bacillus circulans grs313
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ID: 152974
2001
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Abstract
The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
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| Reference Key |
soumen2001brazilianoptimization
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|---|---|
| Authors | ;Palit Soumen;Banerjee Rintu |
| Journal | current drug delivery |
| Year | 2001 |
| DOI |
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| URL | |
| Keywords | Keywords not found |
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