the effects of exercise and conjugated linoleic acid intake on igf-1 and pro-inflammatory cytokines in atrophied skeletal muscle of rats

Background: Conjugated linoleic acid (CLA) can be proposed as an effective nutrient for skeletal muscle atrophy. However, the research related to this is still insufficient. This study was carried out to analyze the mRNA expression of IGF-1 and cytokines in atrophied skeletal muscle of rats. Methods: Forty-two rats were randomly divided into seven groups, each group containing six rats. Sham-Pre and USN-Pre groups underwent a sham operation and a unilateral sciatic nerve (USN) cut, and were sacrificed 1 week later. Other groups had 4 weeks of treatment exercise and CLA intake, and then their blood, liver, and skeletal muscles were sampled after sacrifice. Results: Among the treatment groups, the group treated with both exercise and CLA (USN-EC) showed the lowest body weight. Groups with the sciatic nerve cut showed significantly (p < 0.05) lower muscle weight than groups with the sham operation. However, exercise and CLA intake had no effect on muscle weight. Regarding IGF-1 mRNA, the USN-EC group showed significantly higher expressions in the red muscle of the gastrocnemius and liver than the Sham-Pre and USN-CLA groups. Regarding TNF-α pro-inflammatory cytokine, there was no particular trend; however, the expression of IL-1β mRNA increased in the white muscle of the gastrocnemius muscle and tibialis anterior muscle after sciatic nerve cut, but showed a decrease with exercise and CLA treatment. Particularly in the gastrocnemius white muscle, the group treated with both exercise and CLA showed a significant decrease as compared to groups without treatment after sciatic nerve cut so that positive effects can be expected. Conclusion: It is thought that combining treadmill training with CLA partially influences pro-inflammatory cytokines, so that this can act positively on improving skeletal muscle atrophy caused by sciatic nerve cut.