triploidy determination in rainbow trout (oncorhynchus mykiss) based on erythrocytes dimensions

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ID: 150753
2013
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Abstract
Triploidy induction at fish is characterized by modification of normal diploid chromosome set (2n) into triploid set (3n). Experiments were carried out on biological material from rainbow trout (Oncorhynchus mikiss) during the natural breeding season. Polyploidy was induced by exposing the eggs to heat shock. Blood smear was used as a technical method, to determine diploid and triploid status. Staining of blood smear was performed by Pappenhein method. The erythrocytes area and perimeter measurements done comparatively on triploid and diploid individuals may represent an indicator to determine the ploidy level of individuals. Erythrocytes area (µm²) for triploid individuals is 1,76 times higher than at diploid individuals, and perimeter (µm²) 1,31 for triploid individuals is times higher than in diploid individuals.
Reference Key
bencsik2013scientifictriploidy Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ioan Bencsik
Journal Enzyme and microbial technology
Year 2013
DOI
DOI not found
URL
http://spasb.ro/index.php/spasb/article/view/261
Keywords
heat shock

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