restriction fragment length polymorphism of 195 bp repeated satellite dna of trypanosoma cruzi supports the existence of two phylogenetic groups

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ID: 148635
1999
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Abstract
The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high molecular weight DNA (Hae III), while group 2 presents a ladder profile for each enzyme, which is a characteristic of tandemly repeated DNA. The two groups, respectively, clustered stocks pertaining to the two principal lineages evidenced by isoenzyme and RAPD markers. The congruence among these three independent genomic markers corroborates the existence of two real phylogenetic lineages in T. cruzi. The specific monomorphic profiles for each major phylogenetic lineage suggest the existence of ancient sexuality and cryptic biological speciation.
Reference Key
bastrenta1999memriasrestriction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Brigitte Bastrenta;Marie France Bosseno;Christian Barnabé;Michel Tibayrenc;Simone Frédérique Brenière
Journal kurdistan journal of applied research
Year 1999
DOI
10.1590/S0074-02761999000300008
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000300008
Keywords
trypanosoma cruzi tropical medicinemicrobiology

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