new chromosome numbers in pilocarpus vahl (rutaceae) novos números cromossômicos em pilocarpus vahl (rutaceae)

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2000
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Abstract
Chromosome counts for eight species of Pilocarpus Vahl (Rutaceae) a native of Brazil are reported for the first time. Chromosome numbers were determined from mitotic root tip cells of seedlings derived from field collections and grown in the greenhouse. Feulgen staining was used. Initial pre-treatment of root tips was done by using a saturated aqueous solution of alpha-bromonapthalene for two hours at room temperature (20-25ºC). Chromosome numbers of 2n=44 and 2n=88 were determined for the examined taxa. The present results suggest the occurrence of tetraploidy in P. spicatus St.-Hil. and P. carajaensis Skorupa, and a possible basic number x=22 to the genus Pilocarpus.
Contagens cromossômicas em oito espécies de Pilocarpus Vahl (Rutaceae) do Brasil são relatadas pela primeira vez. Os números cromossômicos foram determinados a partir de células de ponta de raiz de mudas mantidas em casa-devegetação e obtidas em expedições de coleta. O método Feulgen foi empregado para a coloração dos cromossomos. O pré-tratamento das pontas de raiz foi feito em solução saturada de alfa-bromonaftaleno por duas horas à temperatura ambiente (20-25ºC). Foram identificados os números diplóides 2n=44 e 2n=88. Os resultados sugerem a ocorrência de tetraploidia em P. spicatus St.-Hil. e em P. carajaensis Skorupa, e um possível número básico x=22 para o gênero Pilocarpus.
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skorupa2000actanew Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ladislau A. Skorupa
Journal monatshefte für chemie chemical monthly
Year 2000
DOI
10.1590/S0102-33062000000100002
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