new chromosome numbers in pilocarpus vahl (rutaceae) novos números cromossômicos em pilocarpus vahl (rutaceae)
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Abstract
Contagens cromossômicas em oito espécies de Pilocarpus Vahl (Rutaceae) do Brasil são relatadas pela primeira vez. Os números cromossômicos foram determinados a partir de células de ponta de raiz de mudas mantidas em casa-devegetação e obtidas em expedições de coleta. O método Feulgen foi empregado para a coloração dos cromossomos. O pré-tratamento das pontas de raiz foi feito em solução saturada de alfa-bromonaftaleno por duas horas à temperatura ambiente (20-25ºC). Foram identificados os números diplóides 2n=44 e 2n=88. Os resultados sugerem a ocorrência de tetraploidia em P. spicatus St.-Hil. e em P. carajaensis Skorupa, e um possível número básico x=22 para o gênero Pilocarpus.
يُبلّغ للمرة الأولى عن التعدادات الكروموسومية لثمانية أنواع من جنس Pilocarpus Vahl (الفصيلة السذابية Rutaceae) من البرازيل. حُددت الأعداد الكروموسومية انطلاقاً من خلايا قمم الجذور لبادرات حُفظت في دفيئة زراعية وجُمعت خلال بعثات استكشافية حقلية. اعتُمدت طريقة فويلغن لصبغ الكروموسومات. أُجريت المعالجة الأولية لقمم الجذور في محلول مشبع من ألفا-برومونافثالين لمدة ساعتين في درجة حرارة الغرفة (20-25 درجة مئوية). وحُددت الأعداد الضعفانية 2n=44 و2n=88. ترجح النتائج حدوث تضاعف رباعي في P. spicatus St.-Hil. وفي P. carajaensis Skorupa، ووجود عدد أساسي محتمل هو x=22 للجنس Pilocarpus.
| Reference Key |
skorupa2000actanew
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| Journal | monatshefte für chemie chemical monthly |
| Year | 2000 |
| DOI |
10.1590/S0102-33062000000100002
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