experimental tries to establish the preimplantational mammalian embryos viability throughout staining

Clicks: 194
ID: 148386
2007
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Abstract
Presently there are more methods to assess embryo quality but, still the wieldy usedremains the morphological criteria method. In this experiment were tested twostaining methods for embryos and oocytes. The embryos were recovered from mousefemale at 72 hours after mating. The recovered embryos were first evaluated aftermorphological criteria and than by Trypan blue exclusion and Neutral red staining.Using Trypan blue exclusion were evaluated 30 embryos from which 19 (63.3) wereclassified as viable and 11 (36.7) were classified as nonviable. By Neutral redstaining were evaluated 37 embryos from which 24 (64.8) were considered viableand 13 (35.2) were considered nonviable. The oocytes recovered were alsoevaluated using the two methods: using Trypan blue exclusion were stained 10oocytes from which 9 remained uncolored and were considered viable and 1 wasstained in blue and was considered nonviable and using Neutral red 13 oocytes werestained from which 9 were evaluated as viable and 4 as nonviable.
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ivan2007scientificexperimental Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;ALEXANDRA IVAN;ADA TELEA;V. CARABA;N. PACALA
Journal Enzyme and microbial technology
Year 2007
DOI
DOI not found
URL
http://spasb.ro/index.php/spasb/article/view/1434
Keywords Keywords not found

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