detección molecular del virus de la hepatitis e en hígados de cerdo destinados al consumo humano en el estado de nuevo león, méxico molecular detection of hepatitis e virus in pig livers destined for human consumption in the state of nuevo leon, mexico

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ID: 148069
2013
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Abstract
OBJETIVO: Detección molecular del virus de la hepatitis E (VHE) en hígado de cerdo para consumo humano en Nuevo León, México. MATERIAL Y MÉTODOS Se analizaron 127 hígados de cerdo (87 obtenidos de rastros TIF, y 40 de carnicerías) mediante RT-PCR semianidado para amplificar un fragmento de 212 pb del gen ORF2 del VHE. RESULTADOS: El 19.5% (17) de los hígados de rastros y 22.5% (9) de carnicerías fueron positivos. La secuenciación mostró 94-95% de homología con el genotipo 3. CONCLUSIONES: Los resultados indican que el VHE circula en granjas porcinas del estado, lo que constituye una probable fuente de contaminación para los productos cárnicos porcinos.
OBJECTIVE: Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. MATERIALS AND METHODS: 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. RESULTS: 19.54% (17) of tif's and 22.5% (9) of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. CONCLUSIONS: Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.
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cant-martnez2013saluddeteccin Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Marco Antonio Cantú-Martínez;Artur Xavier Roig-Sagués;Sibilina Cedillo-Rosales;Diana Elisa Zamora-Ávila;Ramiro Ávalos-Ramírez
Journal journal of anatomy
Year 2013
DOI
DOI not found
URL
http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0036-36342013000200011
Keywords
mexico hev mexicopublic aspects of medicine

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