in vitro incorporation of helicobacter pylori into candida albicans caused by acidic ph stress

Clicks: 253
ID: 148024
2020
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Abstract
Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions.
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snchez-alonzo2020pathogensin Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Kimberly Sánchez-Alonzo;Cristian Parra-Sepúlveda;Samuel Vega;Humberto Bernasconi;Víctor L. Campos;Carlos T. Smith;Katia Sáez;Apolinaria García-Cancino
Journal computational and mathematical methods in medicine
Year 2020
DOI
10.3390/pathogens9060489
URL
https://www.mdpi.com/2076-0817/9/6/489
Keywords
stress candida albicans ph helicobacter pylori

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