polymorphism within the intron region of the bovine leptin gene in slovak pinzgau cattle

Clicks: 262
ID: 146562
2012
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #56 of 249 articles by views in Enzyme and microbial technology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 249 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Leptin is 16 kDa protein synthesized by adipose tissue and involved energy balance by inhibiting food intake and stimulating energy expenditure, reproductive and immune functions. The physiological properties support leptin as a strong candidate gene for evaluating of genetic polymorphism, which has further been associated with growth, milk yield and other economical traits in livestock. The aim of this study was detection polymorphism in the intron region of the leptin gene on bovine chromosome 4 by using PCR-RFLP method. A total of 100 Slovak Pinzgau cows were used for SNP genotyping. A strategy employing PCR was used to amplify a 422 bp product from hair roots DNA samples. The genotype and allele frequencies for each breed were determined and tested to be in Hardy-Weinberg equilibrium. Digestion of PCR products with restriction enzyme Sau3AI revealed two alleles: allele A was 390, 32 fragments and allele B was 303, 88, 32. Three patterns were observed and frequencies were 0.4761, 0.4278 and 0.0961 for AA, AB and BB, respectively.
Reference Key
moravkov2012scientificpolymorphism Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Nina Moravčíková;Anna Trakovická;Radovan Kasarda
Journal Enzyme and microbial technology
Year 2012
DOI
DOI not found
URL
http://spasb.ro/index.php/spasb/article/view/570
Keywords
cattle pcr-rflp

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.