purification and characterization of beta-1, 3-glucanase from the secretion of simira glaziovii colleters (rubiaceae)
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ID: 146372
2006
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Abstract
In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2
Uma beta-1,3-glucanase foi purificada a partir da secreção de Simira glaziovii, através de vários processos cromatográficos, tendo a análise do perfil protéico acompanhado de SDS-PAGE. A enzima purificada apresentou uma massa molecular estimada de 35 kDa. O pH ótimo obtido para a enzima foi de 5,2.
Uma beta-1,3-glucanase foi purificada a partir da secreção de Simira glaziovii, através de vários processos cromatográficos, tendo a análise do perfil protéico acompanhado de SDS-PAGE. A enzima purificada apresentou uma massa molecular estimada de 35 kDa. O pH ótimo obtido para a enzima foi de 5,2.
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vieira2006brazilianpurification
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|---|---|
| Authors | ;Felipe Almeida Vieira;Maura da Cunha;Denise Espellet Klein;André de Oliveira Carvalho;Valdirene Moreira Gomes |
| Journal | current drug delivery |
| Year | 2006 |
| DOI |
10.1590/S1516-89132006000700004
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