development of an immunochromatographic test strip for the detection of ochratoxin a in red wine

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ID: 146348
2018
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Abstract
Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.
Reference Key
liu2018fooddevelopment Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Liqiang Liu;Liguang Xu;Steven Suryoprabowo;Shanshan Song;Hua Kuang
Journal information security management handbook, fifth edition
Year 2018
DOI
10.1080/09540105.2017.1401043
URL
http://dx.doi.org/10.1080/09540105.2017.1401043
Keywords
Monoclonal antibody mycotoxin ochratoxin a immunochromatographic assay allergy

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