development of an immunochromatographic test strip for the detection of ochratoxin a in red wine
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2018
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Abstract
Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.
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liu2018fooddevelopment
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| Authors | ;Liqiang Liu;Liguang Xu;Steven Suryoprabowo;Shanshan Song;Hua Kuang |
| Journal | information security management handbook, fifth edition |
| Year | 2018 |
| DOI |
10.1080/09540105.2017.1401043
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