measuring enzyme binding using shaped ultrafast laser pulses
Clicks: 101
ID: 145727
2013
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Popular Article
30.0
/100
101 views
17 readers
AI Quality Assessment
Not analyzed
Readership in this journal
PopularRanked #386 of 625 articles by views in utilitas mathematica
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 625 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
We use multiphoton quantum-control spectroscopy to discriminate between enzyme-bound and unbound NADH (reduced nicotinamide adenine dinucleotide) molecules in solution. Shaped ultrafast laser pulses are used to illuminate both forms of NADH, and the ratio of the fluorescence from the bound and unbound molecules for different pulse shapes allows us to measure binding without spectrally resolving the emitted fluorescence or relying on the absolute fluorescence yield. This permits determination of enzyme binding in situations where spectrally resolved measurements and absolute fluorescence yields are difficult to obtain, and makes the approach ideal for multiphoton microscopy with molecular discrimination.
| Reference Key |
c.2013epjmeasuring
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Weinacht T. C.;Tseng C.-H.;Pearson B. J. |
| Journal | utilitas mathematica |
| Year | 2013 |
| DOI |
10.1051/epjconf/20134107013
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.