construction and expression of a synthetic gene encoding nonstructural glycoprotein ns1 of dengue 2 virus in pichia pastoris

Clicks: 379
ID: 145361
2017
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #57 of 145 articles by views in Organic letters

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 145 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris (P. pastoris). Methods: A codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized commercially and inserted into the P. pastoris pPICZαA expression vector. The recombinant DENV-2 NS1 protein was purified by Ni-NTA affinity chromatography, and its antigenicity was tested. Results: The recombinant DENV-2 NS1 protein was secreted as a protein with a molecular weight of ∼45 kDa, and the optimal expression condition was achieved by induction with 2% (v/v) methanol for 72 h. The purified recombinant DENV-2 NS1 protein was able to interact with a monoclonal antibody of NS1 in a commercial rapid test. Conclusions: The resulting recombinant DENV-2 NS1 protein produced in P. pastoris KM71 is a potential candidate for use in the development of a dengue diagnostic kit and vaccine.
Reference Key
puspasari2017asianconstruction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Fernita Puspasari, Riski Dwimalida Putri, Aisyah, Raden Roro Rika Damayanti, Anita Yuwita, Bachti Alisjahbana, Sukwan Handali, Ihsanawati, Dessy Natalia
Are you one of these authors? Claim your profile
Journal Organic letters
Year 2017
DOI
10.1016/j.apjtb.2017.07.017
URL
http://www.sciencedirect.com/science/article/pii/S2221169117307839
Keywords
Dengue virus

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.