quantitative real-time pcr (q-pcr) for sputum smear diagnosis of pulmonary tuberculosis among people with hiv/aids
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2014
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Abstract
Objective: To assess quantitative real-time polymerase chain reaction (q-PCR) for the sputum smear diagnosis of pulmonary tuberculosis (PTB) in patients living with HIV/AIDS with a clinical suspicion of PTB. Method: This is a prospective study to assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV/AIDS with a clinical suspicion of PTB, attended at two referral hospitals for people living with HIV/AIDS in the city of Recife, Pernambuco, Brazil. A Löwenstein-Jensen medium culture and 7H9 broth were used as gold standard. Results: Of the 140 sputum samples, 47 (33.6%) were positive with the gold standard. q-PCR was positive in 42 (30%) of the 140 patients. Only one (0.71%) did not correspond to the culture. The sensitivity, specificity and accuracy of the q-PCR were 87.2%, 98.9% and 95% respectively. In 39 (93%) of the 42 q-PCR positive cases, the CT (threshold cycle) was equal to or less than 37. Conclusion: q-PCR performed on sputum smears from patients living with HIV/AIDS demonstrated satisfactory sensitivity, specificity and accuracy, and may therefore be recommended as a method for diagnosing PTB.
| Reference Key |
albuquerque2014revistaquantitative
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| Authors | ;Yvana Maria Maia de Albuquerque;Ana Luiza Magalhães de Andrade Lima;Ana Kelly Lins;Marcelo Magalhães;Vera Magalhães |
| Journal | 2017 12th international conference for internet technology and secured transactions, icitst 2017 |
| Year | 2014 |
| DOI |
10.1590/S0036-46652014000200009
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