study of callus initiation and growth criteria at different concentrations of 2,4-d and kinetin in taxus baccata l. embryo culture

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ID: 143126
2015
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Abstract
Taxus baccata L. is the main and almost the sole source of paclitaxel, which is an anti neoplastic agent and is used as a cure for treatment of breast and ovary cancer. Since production of this drug is almost a destructive process and yew is an endangered plant, it is required to optimize its tissue culture and micropropagation system. In order to produce embryo derived callus 16 combinations of two plant growth regulators 2,4-D (0, 0.5, 1 and 1.5 mg/L) and Kinetin (0, 0.1, 0.5 and 1 mg/L) were used to study its effects on callus formation and growth in Taxus baccata. It was determined that 1.5 mg/L 2,4-D + 0.1 mg/L kinetin was the best combination for callus growth. Increasing 2,4-D concentration, however, promoted callogenesis while kinetin only in a narrow range had positive effect on callus initiation.
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karimian2015zst/shins-istudy Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ramin Karimian;Mehrdad Lahouti;Mehrdad Lahouti;Seyed Javad Davarpanah
Journal zīst/shināsī-i giyāhī-i Īrān
Year 2015
DOI
DOI not found
URL
http://ijpb.ui.ac.ir/article_18958_6fb374c8cef62129e663868f8e696cbd.pdf
Keywords
4 2 embryo callus kinetinplant culturebiology (general)

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