in vitro propagation of the endangered medicinal orchid, dendrobium lasianthera j.j.sm through mature seed culture
Clicks: 210
ID: 141885
2017
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
210 views
23 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #131 of 145 articles by views in Organic letters
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 145 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Objective: To study asymbiotic seed germination and mass propagation of Dendrobium lasianthera which is one of the endangered medicinal orchids using seeds.
Methods: The 14 weeks old hand pollinated seeds were sown on Vacin and Went (VW) solid medium supplemented with various concentrations of peptone (1, 2, 3 g/L) and without peptone which was used as control treatment. At the 4, 8, and 12 weeks after the seeds were sown, seed germination and shoot formation were investigated. To evaluate the role of organic nutrient additives on subsequent shoot development and root formation, particular shoots with about 1 cm length contains 1–2 leaves obtained from the seeds germination was cultured on VW medium additives with different of organic nutrient: 15% coconut water, 2 g/L peptone, 150 g/L banana homogenate, and without organic nutrient was used as control. After 16 weeks of culture, the plantlet height, number of leaves, number of roots, leaf length and root length were recorded.
Results: The supplementation of 2 g/L peptone in VW medium was proven to be suitable concentration for seed germination (100%) and shoot formation with 84.0% the protocorm development to phase 5 (shoot). VW medium containing 15% coconut water was effectively improved the shoot development, with well developed roots and leaves compared to the other treatment and 95% of acclimatized plantlets survived.
Conclusions: This protocol is an efficient way for the in vitro mass propagation of this Dendrobium lasianthera.
| Reference Key |
utami2017asianin vitro
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Edy Setiti Wida Utami;Sucipto Hariyanto;Yosephine Sri Wulan Manuhara |
| Journal | Organic letters |
| Year | 2017 |
| DOI |
10.1016/j.apjtb.2017.01.011
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.