método fluorescente (diacetato de fluoresceína e brometo de etídeo) para o estudo da viabilidade de cryptococcus neoformans em líquor fluorescein method (fluorescein diacetate and ethidium bromide) to study the viability of cryptococcus neoformans in liquor
Clicks: 229
ID: 139348
1990
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Popular Article
30.0
/100
229 views
45 readers
AI Quality Assessment
Not analyzed
Readership in this journal
PopularRanked #63 of 173 articles by views in 2017 12th international conference for internet technology and secured transactions, icitst 2017
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 173 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Padronizou-se método de fluorescência (solução de diacetato de fluoresceína DF e brometo de etídio BE) para análise de viabilidade de células fúngicas, em 40 amostras de liquor, provenientes de casos comprovados de neurocriptococose. A utilização de solução aquosa de saponina a 0,3% eliminou fluorescências interferentes emitidas por hemácias e leucócitos. Após o processamento dos materiais biológicos, foram retiradas alíquotas de 0,1 ml das supensões obtidas e misturadas a volumes iguais da solução DF-BE preparada pouco antes do uso. O tempo de coloração ideal foi de 30 minutos, resultando perfeita diferenciação entre microrganismos viáveis (fluorescência verde) e não viáveis (fluorescência vermelha).
The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.
The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.
| Reference Key |
corra1990revistamtodo
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Benedito Corrêa;Adhemar Purchio;Claudete R. Paula;Walderez Gambale;Maria Aparecida Shikanai-Yasuda |
| Journal | 2017 12th international conference for internet technology and secured transactions, icitst 2017 |
| Year | 1990 |
| DOI |
10.1590/S0036-46651990000100008
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.