caracterização biológica de onze isolados de pvx (potato virus x) do brasil biological characterization of the pvx isolates (potato virus x) from brazil
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2005
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Abstract
Este trabalho foi realizado com onze isolados do Potato Vírus X (PVX), sendo seis do Centro de Indexação de Vírus Minas Gerais (VIC, LAV, UF, NR, SJ, UDI), um de São Paulo (VEL), dois do Rio Grande do Sul (P21 e P22), um de Santa Catarina (CAN) e um do Distrito Federal (BR). Os isolados de PVX foram inoculados mecanicamente, em duas épocas diferentes (setembro de 2001 e julho de 2002), em hospedeiras diversas, para verificação da sua agressividade e sintomatologia induzida nos hospedeiros, com a finalidade de se fazer sua diferenciação. A diagnose foi feita por inspeção visual e DAS-ELISA. Foram feitas também tentativas de recuperação dos vírus em plantas com DAS-ELISA negativo, por inoculação nas plantas indicadoras Gomphrena globosa e Nicotiana tabacum 'TNN'. Os sintomas induzidos pelos isolados de PVX na maioria das plantas foram semelhantes, com exceção de Nicotiana glutinosa e Physalis floridana, que se revelaram ótimas diferenciadoras para os isolados VEL e P21, respectivamente. Provavelmente os 11 isolados de PVX, estudados neste trabalho, possuem a mesma origem que os isolados dos grupos 1 e 3.
This work was accomplished with eleven PVX isolates: six from national and imported seeds analised at the Virus Indexation Center of Minas Gerais - Brazil (VIC, LAV, UF, NR, SJ, UDI), one from São Paulo state (VEL), two from Rio Grande do Sul state (P21 and P22), one from Santa Catarina state (CAN) and one from the Federal District (BR). The PVX isolates were mechanically inoculated in several hosts plant, in two diferent seasons (September, 2001 and July, 2002), to verify the severity and host symptoms. The diagnosis was done by visual inspection and DAS-ELISA. Attempts to recover the PVX isolates from DAS-ELISA negative plants were also done by inoculation in the indicator plants Gomphrena globosa and Nicotina tabacum TNN. The symptoms shown by the majority of host plants, when inoculated with the eleven isolates, were similar, but Nicotina glutinosa and Physalis floridana showed distinct symptoms when inoculated with P21 and VEL. Probably, the eleven PVX isolates studied in this work have the same origin of the isolates from groups 1 and 3.
This work was accomplished with eleven PVX isolates: six from national and imported seeds analised at the Virus Indexation Center of Minas Gerais - Brazil (VIC, LAV, UF, NR, SJ, UDI), one from São Paulo state (VEL), two from Rio Grande do Sul state (P21 and P22), one from Santa Catarina state (CAN) and one from the Federal District (BR). The PVX isolates were mechanically inoculated in several hosts plant, in two diferent seasons (September, 2001 and July, 2002), to verify the severity and host symptoms. The diagnosis was done by visual inspection and DAS-ELISA. Attempts to recover the PVX isolates from DAS-ELISA negative plants were also done by inoculation in the indicator plants Gomphrena globosa and Nicotina tabacum TNN. The symptoms shown by the majority of host plants, when inoculated with the eleven isolates, were similar, but Nicotina glutinosa and Physalis floridana showed distinct symptoms when inoculated with P21 and VEL. Probably, the eleven PVX isolates studied in this work have the same origin of the isolates from groups 1 and 3.
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| Authors | ;Oneida de Almeida Silva;Antonia dos Reis Figueira;Alessandra de Jesus Boari;César Augusto Brasil Pereira Pinto;Rafael Rodrigues Boni |
| Journal | information and software technology |
| Year | 2005 |
| DOI |
10.1590/S1413-70542005000300003
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