prevalence of virulence genes associated with diarrheagenic pathotypes of escherichia coli isolates from water, sediment, fish, and crab in aby lagoon, côte d’ivoire

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ID: 138248
2017
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Abstract
This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 19 from sediment, and 1 from crab) were positive for virulence genes, including 34 positive for elt (ETEC), 46 positive for est (ETEC), 24 positive for both elt and est, 6 positive for stx (EHEC), 1 positive for both stx + est, and 1 positive for both stx + elt. Genes eae (EPEC) and AggR (EAEC) were not detected. Nine serogroups (O114, O127, O55, O111, O86, O119, O126, O128, and O142) were identified. This study revealed the presence of diarrheagenic and nondiarrheagenic E. coli and potential public health risks if fishery products are not appropriately cooked.
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kambire2017internationalprevalence Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ollo Kambire;Ama Antoinette Adingra;Konan Mathurin Yao;Rose Koffi-Nevry
Journal journal of nanomaterials
Year 2017
DOI
10.1155/2017/9532170
URL
http://dx.doi.org/10.1155/2017/9532170
Keywords
Microbiology

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