hemoculturas para o diagnóstico parasitológico da fase crônica da doença de chagas
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ID: 137862
1975
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Abstract
Os autores apresentam o resultado de hemoculturas de 20 chagásicos crôninos, em meio LIT. Usaram como inóculo o concentrado de células de 10 ml de sangue, lavado em solução fisiológica após remoção do plasma por centrifugação a 3.000r.p.m. A papa, assim obtida, foi semeada em porções iguais em dois tubos contendo, cada um, 5 ml de LIT. Com uma única hemocultura obtiveram 6 casos positivos (30%). Números variáveis de hemoculturas, 1 a 14, nos 20 pacientes, possibilitaram a positividade de 9 casos (45%). Submetendo 7 pacientes a 4 ou mais hemoculturas (4, 5, 6, 10 e 14), 5 dentre eles mostraram-se positivos (71,4%). Recomendam o método para a rotina por ser prático e de fácil execução.
The authors have developed a process to make hemocultures in chronic phase of human Chagas' disease. They used as inocule the washed packed cells of 10 ml of blood. This material was sampled in 2 tubes of LIT (Liver Infusion Tryptose) with 5 ml of the medium. Six positive cases (30%) were detected with only one hemoculture. Increasing the nurriber of hemocultures (1 to 14) 9 out of 20 cases were positives (45%). In 7 cases 4 or more hemocultures (4, 5, 6, 10, 14) were made and 5 of them were positives (71,4%). The authors recomend the method for routine as a practical and easy method
The authors have developed a process to make hemocultures in chronic phase of human Chagas' disease. They used as inocule the washed packed cells of 10 ml of blood. This material was sampled in 2 tubes of LIT (Liver Infusion Tryptose) with 5 ml of the medium. Six positive cases (30%) were detected with only one hemoculture. Increasing the nurriber of hemocultures (1 to 14) 9 out of 20 cases were positives (45%). In 7 cases 4 or more hemocultures (4, 5, 6, 10, 14) were made and 5 of them were positives (71,4%). The authors recomend the method for routine as a practical and easy method
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| Authors | ;Oto G. Mourão;Odeni C. Mello |
| Journal | mobile information systems |
| Year | 1975 |
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