development of primer sets for loop-mediated isothermal amplification that enables rapid and specific detection of streptococcus dysgalactiae, streptococcus uberis and streptococcus agalactiae

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ID: 137360
2015
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Abstract
Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated isothermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as targets, four sets of LAMP primers were designed for identification and differentiation of S. dysgalactiae, S. uberis and S. agalactiae. The detection limit of all four LAMP primer sets were 0.1 pg DNA template per reaction, the LAMP method with 16S rRNA gene and 16S-23S rRNA intergenic spacers as the targets can differentiate the three pathogens, which is potentially useful in epidemiological studies.
Reference Key
wang2015internationaldevelopment Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Deguo Wang;Yanhong Liu
Journal archives of biochemistry and biophysics
Year 2015
DOI
10.3390/ijerph120605735
URL
http://www.mdpi.com/1660-4601/12/6/5735
Keywords
16s rrna gene loop-mediated isothermal amplification (lamp)

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