lipopolysaccharide from crypt-specific core microbiota modulates the colonic epithelial proliferation-to-differentiation balance

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ID: 136330
2017
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Abstract
We identified a crypt-specific core microbiota (CSCM) dominated by strictly aerobic, nonfermentative bacteria in murine cecal and proximal colonic (PC) crypts and hypothesized that, among its possible functions, it may affect epithelial regeneration. In the present work, we isolated representative CSCM strains using selective media based upon our initial 16S rRNA-based molecular identification (i.e., Acinetobacter, Delftia, and Stenotrophomonas). Their tropism for the crypt was confirmed, and their influence on epithelial regeneration was demonstrated in vivo by monocolonization of germfree mice. We also showed that lipopolysaccharide (LPS), through its endotoxin activity, was the dominant bacterial agonist controlling proliferation. The relevant molecular mechanisms were analyzed using colonic crypt-derived organoids exposed to bacterial sonicates or highly purified LPS as agonists. We identified a Toll-like receptor 4 (TLR4)-dependent program affecting crypts at different stages of epithelial differentiation. LPS played a dual role: it repressed cell proliferation through RIPK3-mediated necroptosis of stem cells and cells of the transit-amplifying compartment and concurrently enhanced cell differentiation, particularly the goblet cell lineage.
Reference Key
naito2017mbiolipopolysaccharide Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Tomoaki Naito;Céline Mulet;Cristina De Castro;Antonio Molinaro;Azadeh Saffarian;Giulia Nigro;Marion Béérard;Mééélanie Clerc;Amy B. Pedersen;Philippe J. Sansonetti;Thierry Péééédron;B. Brett Finlay
Journal synlett
Year 2017
DOI
10.1128/mBio.01680-17
URL
http://mbio.asm.org/cgi/content/full/8/5/e01680-17
Keywords
Microbiology

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