development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Clicks: 155
ID: 136271
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Star Article
30.0
/100
155 views
23 readers
AI Quality Assessment
Not analyzed
Readership in this journal
StarRanked #169 of 272 articles by views in mobile information systems
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 272 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Abstract INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. RESULTS: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. CONCLUSIONS: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
| Reference Key |
queirozrevistadevelopment
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Sabrina Ribeiro de Almeida Queiroz;José Valter Joaquim Silva Júnior;Andréa Nazaré Monteiro Rangel da Silva;Amanda Gomes de Oliveira Carvalho;Jefferson José da Silva Santos;Laura Helena Vega Gonzales Gil |
| Journal | mobile information systems |
| Year | Year not found |
| DOI |
10.1590/0037-8682-0220-2017
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.