immobilization of pepsin onto chitosan silica nanobeads with glutaraldehyde as crosslink agent

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ID: 135791
2014
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Abstract

Glutaraldehyde crosslinked chitosan - silica nanobeads have been used as a support in the immobiliza-tion process of pepsin. The effect of pH, pepsin concentration, and temperature on the characteristics of both free and immobilized pepsin had been investigated in this study. The results showed that the im-mobilized pepsin has wider pH range, better capacity, performance and stability to high temperatures compared with those of free pepsin. The immobilized pepsin can be used for 8 to 10 times at which the activity remains 20%, whereas the free pepsin can only be used once. These results suggested promis-ing applications of immobilized enzyme as biocatalyst. © 2014 BCREC UNDIP. All rights reserved.

Received: 1st July 2014; Revised: 3rd September 2014; Accepted: 3rd September 2014

How to Cite: Cahyaningrum, S.E., Sianita, M.M. (2014). Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent. Bulletin of Chemical Reaction Engineering & Catalysis, 9 (3): 263-269. (doi:10.9767/bcrec.9.3.7060.263-269)

Permalink/DOI: http://dx.doi.org/10.9767/bcrec.9.3.7060.263-269

Reference Key
cahyaningrum2014bulletinimmobilization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Sari Edi Cahyaningrum;Maria Monica Sianita
Journal Journal not linked
Year 2014
DOI
10.9767/bcrec.9.3.7060.263-269
URL
http://ejournal.undip.ac.id/index.php/bcrec/article/view/7060
Keywords
chitosan silica

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