identifikasi profil protein oosit kambing pada lama maturasi in vitro yang berbeda dengan sds-page

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ID: 135677
2012
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Abstract
Penelitian tentang identifikasi profil protein oosit kambing pada lama maturasi in vitro yang berbeda dengan SDS-PAGE telah dilakukan. Dari penelitian ini, diharapkan informasi mengenai ekspresi profil protein oosit kambing pada lama maturasi in vitro yang berbeda. Metode penelitian yang digunakan dalam penelitian ini adalah eksperimental labolatoris. Hasil penelitian menunjukkan bahwa profil protein oosit pada lama maturasi in vitro 0,6,12, 18 dan 24 jam adalah : 158 kDa; 116 kDa; 97,2 kDa; 27 kDa dan 6,5 kDa sedangkan pada lama maturasi in vitro 30 jam adalah 116 kDa; 97,2 kDa; 27 kDa dan 6,5 kDa . Disarankan untuk melakukan imunoblotting sebagai konfirmasi untuk mengetahui apakah protein-protein tersebut merupakan protein yang berperan dalam maturasi oosit (yang berperan sebagai Maturation Promoting Factor). Kata kunci : profil protein, oosit kambing, maturasi in vitro, SDS-PAGE IDENTIFICATION OF GOAT OOCYTES PROTEIN PROFIL IN DIFFERENT TIME IN VITRO MATURATION (IVM) BY SDS-PAGE Abstract A study on the identification of goat oocytes protein profile in different time in vitro maturation by SDS-PAGE was conducted. This study was designed as an experimental laboratory study. The results showed that the oocyte protein profile in 0,6,12,18 and 24 hours of IVM were: 158 kDa; 116 kDa; 97,2 kDa; 27 kDa dan 6,5 kDa; while the oocyte protein profile in 30 hours were 116 kDa; 97,2 kDa; 27 kDa and 6,5 kDa. It was suggested to do immunoblotting as confirmation to know that these protein are important in maturation in vitro for goat oocytes Key words : protein profile, goat oocyte, IVM, SDS-PAGE
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Authors ;Nurul Isnaini
Journal jurnal ternak tropika
Year 2012
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