toxicity testing of chitosan from tiger prawn shell waste on cell culture

Clicks: 215
ID: 134629
2009
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Abstract
Background: A biomaterial used in oral cavity should not become toxic, irritant, carcinogenic, and allergenic. Chitosan represents a new biomaterial in dentistry. Purpose: To examine the toxicity of chitosan from tiger prawn shell waste on cell culture with MTT assay. Methods: Chitosan with concentration of 0.25%, 0.5%, 0.75% and 1% was used in this experiment. Each sample was immersed on eppendorf microtubes containing media culture. After 24 hours, the immersion of media culture was used to examine the toxicity effects on BHK-21 cell based on MTT assay method. The density of optic formazan indicates the number of living cells. All data were then statistically analyzed by one-way Anava. Results: The number of living cells in chitosan from tiger prawn shell waste was 93.16%; 85.07%; 78.48%; 75.66%. Thus, there was no significant difference among groups. Conclusion: Chitosan with 0.25%, 0.5%, 0.75% and 1% concentrations from tiger prawn shell waste were not toxic for BHK-21 cell culture when using parameter CD50.
Reference Key
ariani2009dentaltoxicity Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Maretaningtias Dwi Ariani;Anita Yuliati;Tokok Adiarto
Journal industrial crops and products
Year 2009
DOI
10.20473/j.djmkg.v42.i1.p15-20
URL
http://e-journal.unair.ac.id/index.php/MKG/article/view/972
Keywords
toxicity chitosan

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