comparative proteomic analysis of desulfotomaculum reducens mi-1: insights into the metabolic versatility of a gram-positive sulfate and metal-reducing bacterium

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2016
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Ranked #494 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
The proteomes of the metabolically versatile and poorly characterized Gram-positive bacterium Desulfotomaculum reducens MI-1 were compared across four cultivation conditions including sulfate reduction, soluble Fe(III) reduction, insoluble Fe(III) reduction, and pyruvate fermentation. Collectively across conditions, we observed at high confidence ~38% of genome-encoded proteins. Here, we focus on proteins that display significant differential abundance on conditions tested. To the best of our knowledge, this is the first full-proteome study focused on a Gram-positive organism grown either on sulfate or metal-reducing conditions. Several proteins with uncharacterized function encoded within heterodisulfide reductase (hdr)-containing loci were upregulated on either sulfate (Dred_0633-4, Dred_0689-90, and Dred_1325-30) or Fe(III)-citrate-reducing conditions (Dred_0432-3 and Dred_1778-84). Two of these hdr-containing loci display homology to recently described flavin-based electron bifurcation (FBEB) pathways (Dred_1325-30 and Dred_1778-84). Additionally, we propose that a cluster of proteins, which is homologous to a described FBEB lactate dehydrogenase (LDH) complex, is performing lactate oxidation in D. reducens (Dred_0367-9). Analysis of the putative sulfate reduction machinery in D. reducens revealed that most of these proteins are constitutively expressed across cultivation conditions tested. In addition, peptides from the single multiheme c-type cytochrome (MHC) in the genome were exclusively observed on the insoluble Fe(III) condition, suggesting that this MHC may play a role in reduction of insoluble metals.
Reference Key
otwell2016frontierscomparative Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Anne Elyse Otwell;Stephen J Callister;Erika M Zink;Richard D Smith;Ruth E Richardson
Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2016
DOI
10.3389/fmicb.2016.00191
URL
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00191/full
Keywords
sulfate reduction fe(iii) reduction gram-positive bacteria

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