substituição de nitrato de potássio (pa) por salitre potássico no preparo de meio de cultura de tecidos vegetais esterilizado com hipoclorito de sódio potassium nitrate substitution for potassic saltpetre on preparation of plant tissue culture nutritive media esterilized with sodium hypochlorite

Clicks: 281
ID: 133030
2008
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Abstract
A aquisição de nitrato de potássio PA, para qualquer finalidade, depende de autorização do Ministério da Defesa, o que resulta em dificuldades na sua aquisição. Com a finalidade de estudar a possibilidade da substituição desse reagente por outro produto encontrado livremente no comércio, foi conduzido um experimento onde se testaram várias concentrações de salitre potássico em comparação com o nitrato de potássio PA, como componente dos sais de MS. Utilizou-se o produto granulado MURER® nas seguintes concentrações (g L-1): a) 7,0; b) 7,4; c) 7,8; d) 8,2. Foi adicionado um tratamento-controle, com o KNO3 PA, conforme aparece nos sais de MS (1,9 g L-1), sendo a espécie testada à fáfia, a partir de segmentos nodais. O meio de cultura constituiu-se dos sais de MS, vitaminas de White, 30 g L-1 sacarose, 2,0 mg L-1 ácido indol butírico, 2,0 mg L-1 ácido naftalenoacético, 2,0 mg L-1 benzilaminopurina, pH 6,0 + 0,1 e 1,5 g L-1 Phytagel® como gelificante. O número médio de ramos formados para todos os tratamentos foi inferior ao do tratamento-controle; mas, em compensação, o comprimento médio deles foi superior, também em todos os tratamentos, sendo que o comprimento total de ramos na menor concentração de salitre, atingiu 17,0 cm, enquanto no tratamento-controle foi de 12,6 cm. A média da biomassa seca foi de 68,3 mg para a menor concentração de salitre e de 50,0 mg para o tratamento-controle. A análise dos resultados indicou ser vantajosa a substituição do nitrato de potássio PA pelo salitre potássico.
The acquisition of potassium nitrate for any purpose, including preparation of nutritive media for micropropagation, depends on authorization from the Ministry of Defense, what turns out in difficulties in its acquisition. With the purpose of studying the possibility of replacing this component of the culture for another product found freely in the market, an experiment was carried out, where several concentrations of potassic saltpetre were tried, in comparison to the chemically pure potassium nitrate, as a component of the MS salts formulation. The commercial granulated product MURER® was used in the following (g L-1): a) 7,0; b) 7,4; c) 7,8; d) 8,2; and e) chemically pure potassium nitrate (control-treatment) as it appears in the MS salts, or 1,9 g L-1Fáfia was tested, starting from nodal segments. The basal nutrient medium comprised the MS salts, White vitamins, 30 g L-1 sucrose, 2.0 mg L-1 indol butyric acid, 2.0 mg L-1 naftaleneacetic acid, 2.0 mg L-1 benzilaminopurine, pH 6.0 + 0.1 and 1.5 g L-1 Phytagel® as the gelling agent. The number of branches formed in all concentrations of potassic saltpetre was lower than those ones in the control treatment; however, their lengths were higher. The mean length of branches in the treatment with the smallest potassic saltpetre concentration, reached 17.0 cm in length, while in the control-treatment it was 12,6 cm. The dry biomass weight reached 68.3 mg for the smallest potassic saltpetre concentration, but only 50,0 mg for the control treatment. The results indicated to be advantageous the substitution of the chemically pure potassium nitrate for the potassic saltpeter, when either shoot elongation or biomass production is desired, being the nutrient medium sterilized with sodium hipoclorite. However, when a faster multiplication rate is the main objective, the nutrient medium should be prepared with chemically pure potassium nitrate.
Reference Key
ribeiro2008cinciasubstituio Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Juliana Martins Ribeiro;Silvio Lopes Teixeira
Journal information and software technology
Year 2008
DOI
10.1590/S1413-70542008000400026
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400026
Keywords
micropropagation

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