localization of actin in pollen tubes of ornithogalum virens l.

Clicks: 318
ID: 131752
2014
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Abstract
The germinating pollen grain (in vivo on the stigma or in vitro in germination medium) forms a pollen tube which transports the vegetative nucleus and generative cell/two sperm cells participating in the process of double fertilization. The growth of the tube and the transport of organelles and the cells occur due to two major motor systems existing in the pollen tubes of higher plants: the tubuline-dynein/kinesin and the actin-myosin system. In pollen tubes of Ornithogalum virens the actin filaments were labelled with TRITC-phalloidin (2 µg/ml) in the PIPES buffer and the 10% sucrose, without the fixative and DMSO. Omission of the fixative and permeabilizing agent (DMSO) allowed better preservation of the structure, and the "fluorescence" of actin was observed in living pollen tubes. Observations in CLSM (confocal laser scanning microscope) showed that actin is distributed in the vicinity of the cell membrane. This could support the view that actin filaments and the plasmalemma form the pollen tube cortex along which the cytoplasmic movement of organelles, and cell transport occurs.
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stpka2014actalocalization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Małgorzata Stępka;Fabricio Ciampolini;Mauro Cresti;Maria Charzyńska
Journal our dermatol online
Year 2014
DOI
10.5586/asbp.1999.014
URL
https://pbsociety.org.pl/journals/index.php/asbp/article/view/757
Keywords
cortex actin

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