expression of pisum sativum defensin 1 (psd1) in shaking flasks and bioreactor cultivations of recombinant pichia pastoris at different phs
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ID: 130563
2004
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Abstract
The methylotrophic yeast Pichia pastoris was used to produce the recombinant Pisum sativum defensin (rPsd1), a small peptide from pea seeds that has a high level of antifungal activity. The plasmid rPsd1/pPIC9 was integrated into the yeast genome and methanol was used to induce expression and secretion of the recombinant Psd1, at 30º C in a fed-batch mode. The effects of different pH conditions and process scale-up were evaluated using a Monod-type model where dissolved oxygen was considered the limiting substrate. Parameter estimation showed that the process could be improved by expressing rPsd1 in a 1000 mL bioreactor at pH 4. Structural and functional analyses revealed that the recombinant Psd1 is very similar to the native one.
| Reference Key |
larentis2004brazilianexpression
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| Authors | ;A. L. Larentis;M. S. Almeida;K. M. S. Cabral;L. N. Medeiros;E. Kurtenbach;M. A. Z. Coelho |
| Journal | database and network journal |
| Year | 2004 |
| DOI |
10.1590/S0104-66322004000200004
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